Experimental proof of the feasibility of using an angled fiber-optic probe for depth-sensitive fluorescence spectroscopy of turbid media

TitleExperimental proof of the feasibility of using an angled fiber-optic probe for depth-sensitive fluorescence spectroscopy of turbid media
Publication TypePeer Reviewed Archived Journal Publications
Year of Publication2004
AuthorsLiu, Q, Ramanujam N
JournalOpt Lett
Volume29
Pagination2034-6
Date PublishedSep 1
ISBN Number0146-9592 (Print)0146-9592 (Linking)
Accession Number15455771
Keywords*Transducers, Feasibility Studies, Fiber Optic Technology/*instrumentation/methods, Humans, NAD/analysis/diagnostic use, Neoplasms, Glandular and Epithelial/*diagnosis/metabolism, Nephelometry and Turbidimetry/*instrumentation/methods, Optical Fibers, Phantoms, Imaging, Reproducibility of Results, Rhodamines/analysis/diagnostic use, Sensitivity and Specificity, Spectrometry, Fluorescence/*instrumentation/methods, Tumor Markers, Biological/*analysis
Abstract

An angled fiber-optic probe that facilitates depth-sensitive fluorescence measurements was developed for enhancing detection of epithelial precancers. The probe was tested on solid, two-layered phantoms and proved to be effective in selectively detecting fluorescence from different layers. Specifically, a larger illumination angle provides greater sensitivity to fluorescence from the top layer as well as yielding an overall higher fluorescence signal. Monte Carlo simulations of a theoretical model of the phantoms demonstrate that increasing the illumination angle results in an increased excitation photon density and, thus, in increased fluorescence generated in the top layer.

URLhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15455771