|Title||Experimental proof of the feasibility of using an angled fiber-optic probe for depth-sensitive fluorescence spectroscopy of turbid media|
|Publication Type||Peer Reviewed Archived Journal Publications|
|Year of Publication||2004|
|Authors||Liu, Q, Ramanujam, N|
|Date Published||Sep 1|
|ISBN Number||0146-9592 (Print)0146-9592 (Linking)|
|Keywords||*Transducers, Feasibility Studies, Fiber Optic Technology/*instrumentation/methods, Humans, NAD/analysis/diagnostic use, Neoplasms, Glandular and Epithelial/*diagnosis/metabolism, Nephelometry and Turbidimetry/*instrumentation/methods, Optical Fibers, Phantoms, Imaging, Reproducibility of Results, Rhodamines/analysis/diagnostic use, Sensitivity and Specificity, Spectrometry, Fluorescence/*instrumentation/methods, Tumor Markers, Biological/*analysis|
An angled fiber-optic probe that facilitates depth-sensitive fluorescence measurements was developed for enhancing detection of epithelial precancers. The probe was tested on solid, two-layered phantoms and proved to be effective in selectively detecting fluorescence from different layers. Specifically, a larger illumination angle provides greater sensitivity to fluorescence from the top layer as well as yielding an overall higher fluorescence signal. Monte Carlo simulations of a theoretical model of the phantoms demonstrate that increasing the illumination angle results in an increased excitation photon density and, thus, in increased fluorescence generated in the top layer.