Relation between fluorescence spectra of dilute and turbid samples

TitleRelation between fluorescence spectra of dilute and turbid samples
Publication TypePeer Reviewed Archived Journal Publications
Year of Publication1994
AuthorsDurkin, AJ, Jaikumar, S, Ramanujam, N, Richards-Kortum, R
JournalApplied Optics
Volume33
Issue3
Pagination414
Date Published1994
ISSN1539-4522
Abstract

We present a method to extend rank-annihilation-factor analysis (RAFA) for the analysis of fluorescence from homogeneous turbid samples. The method is based on a fundamental relationship between the fluorescence of a dilute solution and that of a turbid solution. We have derived this relationship, known as the transfer function, for turbid materials using the two-flux Kubelka–Munk theory. The method is tested with spectroscopic data from optically thin and turbid samples of the media of a human aorta. At 450-nm excitation, agreement between the measured and predicted dilute-solution fluorescence spectra is within 5% at all emission wavelengths; at 340-nm excitation, agreement is within 20% at all wavelengths, with some residual Soret-band absorption. The simulations presented indicate that the transfer function is markedly more sensitive to absorption than to scattering properties.

DOI10.1364/AO.33.000414
Short TitleAppl. Opt.
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