Morphological Imaging

Project title: Quantitative Diffuse Reflectance Imaging with the Breast Margin Assessment Platform (BMAP)

Project summary: Our Quantitative Diffuse Reflectance Imaging (QDRI) system has the ability to extract optical properties with millimeter resolution from excised breast tissues. The system’s scanning platform allows a comprehensive assessment of an entire breast margin in as fast as 6 minutes, as shown in the figure below. The system’s probe interfaces with the tissue specimen and can gather 16 spectroscopy data points simultaneously. The system’s customized software allows non-experts to collect data through an intuitive interface and employs a fast, robust inversion algorithm to return optical property maps nearly in real time, as shown in Figure 2. Our group has previously determined that cancerous tissue exhibits lower β-carotene concentration and higher reduced scattering coefficients than normal tissue, which we are leveraging for diagnostic contrast. Our system builds upon previous optical surveillance devices of ex vivo breast morphology that have exhibited either a smaller field-of-view, lower resolution, or a data processing time frame unsuitable for intraoperative feedback. These characteristics are necessary to ensure that the surgeon has removed all cancerous tissue before the completion of breast conserving surgeries. Failure to do so requires a re-excision, which happens to almost 1 in 4 patients with early stage breast cancer. Our laboratory is currently gathering intraoperative clinical data at Duke University Hospital and University of Wisconsin-Madison Hospital (starting January 2015) to validate our diagnostic algorithm and ultimately reduce the re-excision rate for patients with breast cancer.

The Breast Margin Assessment Platform (BMAP). Our three-dimension translation QRDI Imaging platform with 16 channel probe is shown at left. a) Digital photo showing sites inked for pathological review, b), raster-scanned reflectance map averaged from 450–600nm, c), hi-resolution optical property map of wavelength-averaged μs’, d), corresponding map representing [β-carotene], and e), corresponding ratio map of [β-carotene] to μs’. Sites labeled 1–6 contain mostly fat, sites 7–10 are mostly fibro-glandular tissue.

Collaboration: If you are are interested in working with us to use this technology at your site, please contact Jennifer Gallagher (